Oroxylum indicum (L.) extract protects human neuroblastoma SH‑SY5Y cells against β‑amyloid‑induced cell injury

Oroxylum indicum (L.) extract protects human neuroblastoma SH‑SY5Y cells against β‑amyloid‑induced cell injury


English :

It has been reported that amyloid β peptide, the
major component of senile plaques, serves a critical role in
the development and progression of Alzheimer's disease
(AD ) by generating reactive oxygen species (RO S), leading to
oxidative stress. The aim of the present study was to investigate
the protective effect of Oroxylum indicum (L.) extract
against Aβ25‑35‑induced oxidative stress and cell injury using
SH‑SY5Y cells as a model, and at exploring the underlying
mechanisms. The results revealed that the exposure of cells
to 20 μM Aβ25‑35 significantly increased cellular oxidative
stress, as evidenced by the increased RO S levels. Aβ25‑35
treatment also increased caspase‑3/7 activity and lactate
dehydrogenase (LD H) release, and caused viability loss.
Oroxylum indicum treatment not only attenuated the generation
of RO S and suppressed caspase‑3/7 activity but also reduced
the neurotoxicity of Aβ25‑35 in a concentration‑dependent
manner, as evidenced by the increased cell viability and
decreased LD H release. Treatment with Oroxylum indicum
also increased superoxide dismutase (SOD ) and catalase
(CA T) activity, increased the phosphorylation of Akt and
cAMP‑responsive element binding protein (CRE B), and
contributed to the upregulation of Bcl‑2 protein. In combination,
these results indicated that Oroxylum indicum extract
could protect SH‑SY5Y cells against Aβ25‑35‑induced cell
injury, at least partly, by inhibiting oxidative stress, increasing
SOD and CA T activity, attenuating caspase 3/7 activity and
promoting the cell survival pathway, Akt/CRE B/Bcl‑2. The
approach used in the present study may also be useful for
preventing the neurotoxicity induced by Aβ in AD and related