Purpose: To investigate the anti-inflammatory effects and antioxidant activity of Oroxylum indicum (L.)
Kurz fruit extract in lipopolysaccharide (LPS)-stimulated BV2 microglia.
Methods: BV2 cells were treated with LPS for 24 h in the presence or absence of O. indicum fruit
extract. Then, nitric oxide (NO), reactive oxygen species (ROS) and interleukin 6 (IL-16) levels were
measured using Griess reagent assay, CM-H2DCFDA and enzyme-linked immunosorbent (ELISA)
assays, respectively. The in vitro antioxidant property of the extract was also investigated by 1,1-
diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS)
Results: Levels of IL-6, NO, and ROS in LPS-treated BV2 cells were significantly higher than those in
control (p < 0.01). However, exposure of LPS-treated BV2 cells to O. indicum extract led to a marked
decrease in the levels of these parameters, when compared to the untreated cells (p < 0.01). Results
from DPPH and ABTS assays showed that the O. indicum extract exhibited good antioxidant properties,
with total flavonoid and total phenolic contents of 115.58 ± 1.09 and 131.04 ± 2.37 mg/g of dried extract,
Conclusion: The results demonstrate that O. indicum fruit exerts anti-oxidant and anti-inflammatory
effects in LPS-stimulated BV2 cells. Thus O. indicum fruit might be beneficial in the development of
novel anti-oxidative and anti-neuroinflammatory herbal medicines. However, the mechanisms by which
O. indium fruits reduces NO and IL-6 needs to be further investigated.